Restriction Enzymes

Restriction enzymes cleave DNA into fragments at or near specific recognition sequences known as restriction sites; also known as restriction endonuclease, or restrictase. Products include various restriction enzymes, kits, buffers, etc.

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Thermo Scientific™ BpiI (BbsI)

Cut GAAGAC(2/6)^ sites with BpiI (BbsI) restriction enzyme, which performs best at 37°C in G buffer (Isoschizomers: BbsI, BpuAI, BstV2I).

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Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	BpiI (BbsI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer G
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentrazione	10 U/μL

Thermo Scientific™ OliI (AleI) (10 U/μL)

The OliI (AleI) restriction enzyme recognizes CACNN^NNGTG sites and cuts best at 37°C in R buffer (Isoschizomers: AleI).

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	OliI (AleI)
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer R
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentrazione	10 U/μL

Thermo Scientific™ FastDigest Eco31I (IIs class)

The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Eco31I
Methylation Sensitivity	CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Sporgenza	5' protruding ends
Inactivation Temperature	65°C
Product Line	FastDigest™

Thermo Scientific™ Esp3I (BsmBI)

The Esp3I (BsmBI) restriction enzyme recognizes CGTCTC(1/5)^ sites and cuts best at 37°C in Tango (+DTT) buffer (Isoschizomers: BsmBI).

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	Esp3I (BsmBI)
Methylation Sensitivity	CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category	Scarless Cloning
Compatible Buffer	10X Buffer Tango
Type IIS RE	Yes
Optimal Reaction Temperature	37°C
Concentrazione	10 U/μL

Thermo Scientific™ MssI (PmeI)

The MssI (PmeI) restriction enzyme recognizes GTTT^AAAC sites and cuts best at 37°C in B buffer (Isoschizomers: PmeI).

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Sensitive to Heat Inactivation	Yes
Product Type	Restriction Enzyme
Enzyme	MssI (PmeI)
Methylation Sensitivity	Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive
Research Category	Traditional Cloning
Compatible Buffer	10X Buffer B
Type IIS RE	No
Optimal Reaction Temperature	37°C
Concentrazione	5 U/μL

Thermo Scientific™ Tampone tango 10x

Ensure the optimum reaction conditions for restriction enzymes with Thermo Scientific™ 10X Buffer Tango, premixed with BSA for enhanced stability.

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Thermo Scientific™ SmaI

Cut at CCC^GGG sites with SmaI restriction enzyme, which performs best at 30°C in Tango buffer (Isoschizomers: TspMI, XmaCI, XmaI).

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/μL

Thermo Scientific™ XhoI

Cut at C^TCGAG sites with XhoI restriction enzyme, which functions best at 37°C in R buffer (Isoschizomers: PaeR7I, Sfr274I, SlaI, StrI, TliI).

Promozioni disponibili

Prezzi e disponibilità
Specifiche

Concentrazione	10 U/μL

Restriction Enzymes

Restriction Enzymes

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